建立了液相色谱-串联质谱法测定人血浆中的匹维溴铵。以紫杉醇为内标，采用C18色谱柱，流动相为乙腈-2 mmol/L乙酸铵溶液-甲酸(70︰30︰0.1)，多反应监测(MRM)扫描，监测离子对m/z 512.5→231.3(匹维溴铵)和m/z 854.3→286.3(紫杉醇)。低、中、高浓度匹维溴铵的血浆提取回收率分别为111.7％、104.9％和99.7％，日内和日间RSD均小于15％。

A LC-MS/MS method was established for the determination of pinaverium in human plasma. Paclitaxel was used as the internal standard. A C18 column was used with the mobile phase of acetonitrile-2 mmol/L ammonium acetate-formic acid (70︰30︰0.1) in multiple reaction monitoring (MRM) mode with the transitions of m/z 512.5→231.3(pinaverium) and m/z 854.3→286.3(paclitaxel), respectively. The recoveries of pinaverium at low, medium and high concentration levels were 111.7％, 104.9％ and 99.7％, respectively. The inter- and intra-day RSDs were less than 15％.