用过氧化氢氧化还原型谷胱甘肽制备了氧化型谷胱甘肽，并优化反应条件为：还原型谷胱甘肽浓度200 ～ 300 g/L，反应pH 5.0 ～ 6.0，过氧化氢终浓度3％。可得纯度99.5％的氧化型谷胱甘肽，总收率为95.9％。考察了产物对对乙酰氨基酚诱导的小鼠急性肝损伤的治疗作用，结果显示能显著降低谷丙转氨酶和谷草转氨酶的活性，且与还原型谷胱甘肽没有显著性差异。
Abstract
Reduced glutathione was oxidized by hydrogen peroxide to obtain oxidized glutathione. The optimal process was as follows: the reduced glutathione in 200 - 300 g/L was reacted with 3％ hydrogen peroxide at the pH value of 5.0 - 6.0. The purity and total yield of oxidized glutathione after purification were 99.5％ and 95.9％, respectively. Furthermore, the therapeutic effect of oxidized glutathione on acute liver injury in mice induced by paracetamol was investigated. The results showed that it could significantly decrease the activities of glutamate pyruvate transaminase and glutamic-oxal(o)acetic transaminase, and there was no significant difference between oxidized glutathione and the reduced form.
关键词
氧化型谷胱甘肽 /
制备 /
急性肝损伤
{{custom_keyword}} /
Key words
oxidized glutathione /
preparation /
acute liver injury
{{custom_keyword}} /
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] Valko M, Leibfritz D, Moncol J, et al. Free radicals and antioxidants in normal physiological functions and human disease [J]. Int J Biochem Cell Biol, 2007, 39(1): 44-84.
[2] 袁平戈, 张大志. 还原型谷胱甘肽的作用机制及临床应用[J]. 药品评价, 2006, 3(5): 385-390.
[3] 胡学建, 胡剑浩, 陈龙根. 还原型谷胱甘肽修复受损肝细胞功能疗效观察[J]. 实用医学杂志, 2006, 22(1): 46-47.
[4] 于春虎, 高 阳. 阿拓莫兰在化疗中护肝作用评价[J]. 药品评价, 2008, 5(7): 313-314.
[5] 卢永科, 川岛明, 堀井郁夫, 等. 顺铂对大鼠肝细胞毒性及谷胱甘肽的保护作用[J]. 中国公共卫生, 2004, 20(4): 440-441.
[6] 刘淑珍, 冉 华, 周丽霞. 还原性谷胱甘肽治疗药物性肝损害38 例临床分析[J]. 山西医药杂志: 下半月刊, 2008, 37(3): 236-237.
[7] 孙经利, 魏新泉, 张龙跃, 等. 还原型谷胱甘肽联合甘利欣治疗急性肝炎120 例临床观察[ J] . 临床肝胆病杂志, 2006, 22(3): 181.
[8] 金春英, 崔京兰, 崔胜云. 氧化型谷胱甘肽对还原型谷胱甘肽清除自由基的协同作用[J]. 分析化学, 2009, 37(9): 1349-1353.
[9] 刘中兴, 谢传欣, 石 宁, 等. 过氧化氢溶液分解特性研究[J]. 齐鲁石油化工, 2009, 37(2): 99-102.
[10] Lu SC. Regulation of glutathione synthesis [J]. Mol Aspects Med, 2009, 30(1-2): 42-59.
[11] Tokuichi T, Tomoko M, Iwao S. Glutathione-containing drink: JP, 05146279A [P]. 1993-06-15.
[12] Susumu S, Kunihide N, Katsuyuki K, et al. Production of oxidized glutathiont ascorbic acid-oxidizing enzyme: JP, 07177896A [P]. 1995-07-18.
[13] K o z h e m y a k i n L A , B a l a s o v s h i M B . M e t h o d s f o r production of the oxidized glutathione composite with CISdiamminedichloroplatinum
and pharmaceutical compositions based thereof regulating metabolism, proliferation, differentiation and apoptotic mechanisms for normal and transformed cells: US, 20070142267 [P]. 2007-06-21.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
