建立了超高效液相色谱串联质谱法测定大鼠灌胃给予参松养心胶囊后胆汁中的芍药苷、斯皮诺素和五味子甲素。采用Waters UPLC C18 色谱柱，乙腈-0.1％甲酸为流动相，梯度洗脱，正离子多反应监测(MRM)。芍药苷、斯皮诺素和五味子甲素分别在24.6 ～ 1 720、28.6 ～ 1 430 和2.8 ～ 700 ng/ml 范围内线性关系良好，提取回收率为88.7％～100.9％，基质效应为91.28％～ 102.70％，日内和日间RSD 均小于6.22％，相对误差为-6.58 ～ 7.51％。

An UPLC-MS/MS method was established for the determination of paeoniflorin, spinosin and deoxyschizandrin in rat bile after intragastric administration of Shensong Yangxin capsules. A Waters UPLC C18 column was used with the mobile phase of acetonitrile-0.1％ formic acid by gradient elution. Electrospray ionization (ESI) source was employed and the three analytes were detected by multiple reaction monitoring (MRM) scanning under the positive ionization mode. The calibration curves for paeoniflorin, spinosin and deoxyschizandrin were linear in the concentration ranges of 24.6 - 1 720, 28.6 - 1 430 and 2.8 - 700 ng/ml, respectively. Their extraction recoveries were 88.7％ - 100.9％ and the matrix effects were 91.28％ - 102.70％ in rat bile. The intra- and inter-day RSDs were less than 6.22％ with the relative errors (RE) within -6.58％ to 7.51％.