建立了高效液相色谱法测定栀子苷和栀子苷元的血浆蛋白结合率。采用C18 色谱柱，流动相为乙腈-0.05％磷酸( 15 ︰ 85)，检测波长238 nm。测得栀子苷、栀子苷元与小牛血清白蛋白、人血清白蛋白和大鼠血浆的蛋白结合率分别为(38.1±4.2)％和(63.4±1.2)％，(17.6±4.9)％和(81.5±6.2)％，以及(24.5±3.8)％和(94.6±1.0)％。结果表明栀子苷元与血浆蛋白的结合力高于栀子苷。
Abstract
An HPLC method was established for the determination of plasma protein binding rates of geniposide and genipin. A C18 column was used with the mobile phase of acetonitrile-0.05％ phosphoric acid(15︰85) at the detection wavelength of 238 nm. The plasma protein binding rates of geniposide and genipin with BSA, HSA and rat plasma were (38.1±4.2)％ and (63.4±1.2)％, (17.6±4.9)％ and (81.5±6.2)％, (24.5±3.8)％ and (94.6±1.0)％, respectively. The results showed that the binding capacity of genipin with the above plasma proteins was higher than that of geniposide.
关键词
栀子苷 /
栀子苷元 /
血浆蛋白结合率 /
高效液相色谱 /
测定
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Key words
geniposide /
genipin /
plasma protein binding rate /
HPLC /
determination
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参考文献
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脚注
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基金
国家自然基金(81173561)、教育部新世纪人才计划(NCET-10-0944)、上海市教委重点学科(J50302)、上海市教委科研基金(2010JW21)
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