建立了虎杖药渣中白藜芦醇的含量测定方法和提取纯化工艺。以白藜芦醇含量为评价指标，采用正交试验优化提取工艺。以大孔吸附树脂、柱色谱及重结晶法分离并纯化提取物浸膏中的白藜芦醇。结果表明，最佳提取工艺为虎杖药渣用90％乙醇加热至85 ℃回流提取3 次，每次料液比均为1 ∶ 10，提取时间分别为1.5、1 和0.5 h。所得提取物浸膏用水溶解后经D101 型大孔吸附树脂吸附富集、硅胶柱色谱纯化和丙酮重结晶，可获得纯度达99％的白藜芦醇。

The determination, extraction and purification of resveratrol from herbal residues of Polygonum cuspidatum Sieb. et Zucc. were investigated. The extraction process was optimized by orthogonal design with the content of resveratrol as index. The resveratrol in extract was separated by macroporous adsorptive resin and chromatography and then purified by recrystallization. The optimal extraction process was as follows: the herbal residues were extracted by 90％ ethanol with solid-liquid ratio of 1∶10 at 85 ℃ (reflux) for three times in 1.5, 1 and 0.5 h, respectively. The resveratrol in the extract dissolved in water was enriched by D101 macroporous adsorptive resin, followed with separation by silica column chromatography and recrystallization in acetone. The purity of final product was above 99％.