建立了暴露于空气 - 液体界面 (ALI) 培养模式下的人肺泡Ⅱ型上皮细胞系 A549- 炎症损伤模型，采用脂多糖 (LPS) 作为炎性刺激因子，探讨其在不同质量浓度 (0、10、30、50 μg/ml) 和不同作用时间 (1、3、5 h) 下对白细胞介素 (IL-1β、 IL-6、IL-8、IL-18)、肿瘤坏死因子 -α、转化生长因子 -β 基因表达的影响。结果显示，LPS 作用 1 h 时，除 IL-6 外，其余各剂量组均诱导上述炎症因子表达增加。炎症因子的表达与 LPS 的质量浓度无关，与其作用时间有关，为确定 ALI 培养炎症细胞模型的最佳质量浓度和时间提供了试验基础。
Abstract
A human type Ⅱ alveolar epithelial cell line A549-inflammation injury model exposed to air-liquid interface(ALI) cultivation mode was established using lipopolysaccharide(LPS) as an inflammatory stimulator. The effects of different mass concentrations(0, 10, 30, 50 μg/ml) and durations(1, 3, 5 h) of LPS on the gene expression of interleukins(IL-1β, IL-6, IL-8, IL-18), tumor necrosis factor-α and transorming growth factor-β were investigated. The results showed that the expressions of the above-mentioned inflammatory factors were increased in all dose groups except IL-6 when LPS acted for 1 h. The expressions of inflammatory factors were independent of the mass concentration of LPS, but related to its action time, which provided an experimental basis for determining the optimal concentration and time of ALI cultured inflammatory cell model.
关键词
A549 细胞 /
空气 - 液体界面培养 /
肺部炎症模型 /
脂多糖
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Key words
A549 cell /
air-liquid interface culture /
pulmonary inflammation model /
lipopolysaccharide
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