在炎症反应中，人的T 淋巴细胞及其分泌的细胞因子起到关键作用。近年来黄酮类化合物的抗炎作用日益受到人们关注，灯盏乙素苷元( 1) 和二氢杨梅素( 2) 都是天然植物中提取的黄酮类化合物，具有较好抗炎作用和开发潜力。本试验首先在体外成功培养扩增了人CD3+ T 淋巴细胞，然后考察了CD3+ T 淋巴细胞分别经1 和2 处理后自身细胞活性和凋亡情况，以及表面CD25 和CD69 的表达和白介素(IL)-17A、IL-6、肿瘤坏死因子α(TNF-α)、干扰素γ(INF-γ) 的分泌水平。结果表明，1 和2 都能明显抑制TNF-α 的分泌，在40 μmol/L 时，TNF-α 的分泌水平分别只有空白对照组的 54.5％和69.3％；二者对其他细胞因子的分泌无明显影响。CCK-8 检测结果显示1 和2 对CD3+ T 淋巴细胞无明显细胞毒性，细胞存活率均高于80%。并且，1 和2 对CD3+ T 淋巴细胞的凋亡及其表面CD25 和CD65 的表达也无明显影响。总的来说， 1 和2 能直接抑制CD3+ T 淋巴细胞分泌炎症因子TNF-α，同时并不会影响CD3+ T 淋巴细胞的细胞活性和功能活性。
Abstract
Human T lymphocytes and their secreted cytokines play key roles in the inflammatory response. The anti-inflammatory effect of flavonoids has attracted more and more attention in recent years. Scutellarein(1) and dihydromyricetin(2) are classical flavonoids extracted from natural herbs, which have good anti-inflammatory effect and development potential. In this paper, human CD3+ T lymphocytes were successfully cultured and expanded in vitro. Then, the activity and apoptosis of human CD3+ T lymphocytes after treatment with 1 or 2 were investigated. The effects of 1 and 2 on the expressions of CD25 and CD69 on the lymphocytes and levels of interleukin(IL)-17A, IL-6, tumor necrosis factor α(TNF-α) and interferon-γ(INF-γ) secreted by the lymphocytes were also determined. The results showed that 1 and 2 significantly inhibited the secretion of TNF-α. The secretion levels of TNF-α in the groups of 1 and 2 both at 40 μmol/L were 54.5％ and 69.3％ of the amount in the blank control group, while both 1 and 2 had no significant inhibitions on other cytokines. The results of CCK-8 test showed that the survival rates of CD3+ T lymphocytes after treatment with 1 or 2 were above 80％. Moreover, there were no significant changes in apoptosis and expressions of CD25 and CD65 between the 1 or 2 groups and the control groups. It was concluded that the flavonoids 1 and 2 could directly inhibit the secretion of TNF-α by CD3+ T lymphocytes, but they did not affect the cellular and functional activities of CD3+ T lymphocytes.
关键词
人CD3+ T 淋巴细胞 /
黄酮类化合物 /
灯盏乙素苷元 /
二氢杨梅素 /
抗炎作用
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Key words
human CD3+ T lymphocyte /
flavonoids /
scutellarein /
dihydromyricetin /
anti-inflammatory effect
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