本研究通过开发高效的脱色工艺和精细纯化工艺，获得了高质量的替考拉宁，缩短了生产时间。通过对比活性炭和阴离子交换树脂的脱色效果，开发聚酰胺解吸液( 替考拉宁含量3.8 g/L) 的脱色工艺。结果显示，阴离子交换树脂的脱色效果更优。通过对比HP20SS、聚合物微球(UniPS 40-300) 和色谱8 号3 种不同型号的色谱介质的分离效果，开发替考拉宁的精细纯化工艺。结果显示，选用色谱8 号为色谱介质时效果最优，色谱条件如下：上样量16 ～ 20 g/L，洗脱溶剂为乙醇∶ 0.05％磷酸二氢钾溶液(50 ∶ 50)(pH 5.0)，洗脱速度为1 ～ 2 BV/h ；纯化所得产品平均纯度为99.9％，纯化收率≥ 90％。该工艺较目前国内企业普遍的8 ～ 10 d 的生产周期缩短了50% 以上，仅需4 d。
Abstract
A decolorization and fine purification process was developed to get high-quality teicoplanin and reduce the production cycle. The decolorization process of the eluent from polyamide column (teicoplanin content 3.8 g/L) was developed by comparing the decolorization efficiencies of activated carbon and anion-exchange resin. The results showed that the latter was much better. The fine purification process of teicoplanin was developed by comparing the separation efficiencies of three types of chromatographic separating medium, namely HP20SS, polymer microspheres UniPS 40-300 and chromatography No.8. Finally, chromatography No.8 was selected as the chromatographic separating medium with the following chromatographic conditions: ethanol∶0.05％ potassium dihydrogen phosphate solution (50∶50)(pH 5.0) as the eluent, the loading amount of 16 - 20 g sample on 1 L chromatographic separating medium, at the flow rate of 1 - 2 BV/h. The product obtained had a purity of 99.9％, with the yield no less than 90％. In addition, the production cycle (only 4 days) of this process was shortened by more than 50％, compared with the existing production cycle of 8 - 10 d in domestic manufacturers.
关键词
替考拉宁 /
脱色 /
色谱 /
洗脱 /
纯化
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参考文献
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