建立了高效液相色谱法测定壮药八味龙钻颗粒中的青藤碱、木兰花碱、橙皮苷和毛两面针素。采用Capcell PAK C18 色谱柱，流动相为乙腈∶ 0.2％磷酸，线性梯度洗脱，检测波长230 nm。青藤碱、木兰花碱、橙皮苷和毛两面针素分别在34.4 ～ 172.0、30.4 ～ 152.0、18.4 ～ 92.0 和26.0 ～ 130.0 μg/ml 范围内线性关系良好，回收率分别为96.49％、 103.44％、98.80％和99.02％，RSD 分别为2.27％、1.48％、3.58％和2.61％。该方法简便、准确，重现性好，可用于八味龙钻颗粒中4 种有效成分的含量测定，并为其质量控制提供参考。
Abstract
An HPLC method was established for the determination of four active ingredients, sinomenine, magnoflorine, hesperidin, and toddalolactone in Bawei Longzuan granules, a Zhuang folk medicine. A Capcell PAK C18 column was used, with the mobile phase of acetonitrile∶0.2％ phosphoric acid by gradient elution, at the detection wavelength of 230 nm. The calibration curves of sinomenine, magnoflorine, hesperidin, and toddalolactone were linear in the ranges of 34.4-172.0, 30.4-152.0, 18.4-92.0, and 26.0-130.0 μg/ml, respectively. Their recoveries were 96.49％, 103.44％, 98.80％ and 99.02％, with RSDs of 2.27％, 1.48％, 3.58％ and 2.61％, respectively. The developed HPLC method was successfully applied to the simultaneous determination of the four compounds in Bawei Longzuan granules. The results indicated that the established method was simple, accurate and reproducible for the determination of the four active ingredients, and it could be a promising method for the quality control of Bawei Longzuan granules.
关键词
八味龙钻颗粒 /
青藤碱 /
木兰花碱 /
橙皮苷 /
毛两面针素 /
高效液相色谱法 /
含量测定
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Key words
Bawei Longzuan granule /
sinomenine /
magnoflorine /
hesperidin /
toddalolactone /
HPLC /
content determination
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参考文献
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脚注
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基金
广西省教育厅广西高校壮医方药基础与应用研究重点实验室项目(桂教科研〔2016〕6号zyfy201613)
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