建立了高效液相色谱法测定依折麦布片的有关物质。采用Hypersil Gold C8 色谱柱，以甲醇∶ 0.05％三氟乙酸溶液(50 ∶ 50) 为流动相，检测波长232 nm。依折麦布与其非对映异构体[6，(3R,4S)-1-(4- 氟苯基)-3-[(3R)-3-(4- 氟苯基)-3-羟基丙基] -4-(4- 羟基苯基) -2- 氮杂环丁酮] 的分离度为1.8；依折麦布及其有关物质2、非对映异构体和有关物质3 分别在12 ～ 820、9.6 ～ 640、12 ～ 780 和12 ～ 780 ng/ml 范围内线性关系良好。上述有关物质的相对校正因子分别为0.8、1.0 和1.2；平均回收率分别为102.5％、104.4％和97.9％，RSD 分别为1.3％、1.7％和2.4％。

An HPLC method was established for the determination of related substances in ezetimibe (1) tablets. A Hypersil Gold C8 column was used, with the mobile phase of methanol∶0.05％ TFA (50∶50), at the detection wavelength of 232 nm. The resolution between 1 and its diastereoisomer [6, (3R,4S)-1-(4-fluorophenyl)-3-[(3R)-3-(4-
fluorophenyl)-3-hydroxypropyl]-4-(4-hydroxyphenyl)azetidin-2-one] was 1.8. It was linear for 1, the related substance 2, diastereoisomer 6 and the related substance 3 in the ranges of 12—820, 9.6—640, 12—780 and 12—780 ng/ml, respectively. Their relative correction factors were 0.8, 1.0 and 1.2. Their average recoveries were 102.5％, 104.4％ and 97.9％, with RSDs of 1.3％, 1.7％ and 2.4％, respectively.