SHEN Jinglin1, DING Ying2
An HPLC-wavelength switching method was established for the determination of allantoin (1),dehydrotumulosic acid (2), tumulosic acid (3), pachymic acid (4), catalpol (5), jionoside B1 (6), martinoside (7),ginsenoside Rg1 (8) and ginsenoside Rb1 (9) in Qinggong Changchun Jiaonang. An Agilent TC-C18 column was used,with the mobile phase of acetonitrile∶0.2％ phosphoric acid by gradient elution, at the detection wavelength of 224 nm(0→19 min, 1), 210 nm (19→31 min, 2—5), 330 nm (31→37 min, 6 and 7) and 203 nm (37→55 min, 8 and 9). Their calibration curves were linear in the ranges of 8.0—160.0, 2.0—40.0, 2.5—50.0, 2.0—40.0, 5.0—100.0, 2.5—50.0,2.0—40.0, 15.0—300.0 and 8.5—170.0 μg/ml, respectively. Their average recoveries were 98.82％, 100.03％, 97.92％,98.09％, 97.64％, 98.14％, 96.91％, 97.88％ and 98.39％, with RSDs less than 2％. This method can be used for the quality analysis of Qinggong Changchun Jiaonang.
