Paper
CHEN Hui # , ZHANG Fen# , WANG Jianan, WANG Qingfang, CHU Wanglong
The study systematically compared the performance differences between domestic and imported culture
media in the cultivation of mesenchymal stem cells(MSCs). Three distinct culture systems were employed to culture
MSCs, and key indicators including cell morphology, cell surface markers, differentiation potential, immunomodulatory
capacity, karyotype analysis, cellular senescence, and soft agar colony formation were comprehensively evaluated to assess
the safety, efffcacy, and cell yield of MSCs in different culture systems. The results demonstrated that MSCs cultured in all
three systems exhibited the typical spindle-shaped morphology, with cell surface markers conforming to international stem
cell standards, and possessed osteogenic, adipogenic, and chondrogenic differentiation potential. The karyotype was normal,
and soft agar colony formation assays conffrmed the absence of tumorigenic risk. The results indicated that there was no
signiffcant difference between domestic and imported culture media. However, the domestic culture medium demonstrated
certain advantages in some aspects: it had stronger cell proliferation ability and higher passage stability. In terms of
immunomodulatory function, the domestic medium exhibited stronger inhibitory effects on T lymphocyte suppression and
TNF-α. Cellular senescence assays showed a lower senescence rate in the domestic culture system. Additionally, when the
domestic medium was used as a cryopreservation solution component, cells demonstrated faster recovery of viability postthawing.
This study provides scientiffc evidence to advance the domestic production of culture media in China, thereby
enhancing the country’s independent innovation capabilities and international competitiveness in the field of stem cell
research and application.
