As environmentally benign and sustainable technologies, enzyme immobilization and continuous flow synthesis have been widely applied to organic synthesis. In recent years, immobilized enzyme based flow biocatalysis has received increasing attention and application, because it possesses the integrated advantages of enzyme immobilization and continuous flow synthesis. Ketoreductase-catalyzed asymmetric reduction of prochiral ketones is one of the most effective methods for the synthesis of chiral alcohols. This paper summarizes the research progress of immobilized ketoreductase in continuous flow synthesis, and provides guidance for developing efficient, economic, and sustainable flow biocatalysis approaches.