Review
Hu Pan, Wu Xiaofan, Huang Zedu, Chen Fener,
Chinese Journal of Pharmaceuticals.
Accepted: 2024-12-06
As environmentally benign and sustainable technologies, enzyme immobilization and
continuous flow synthesis have been widely applied to organic synthesis. In recent years, immobilized
enzyme based flow biocatalysis has received increasing attention and application, because it possesses the
integrated advantages of enzyme immobilization and continuous flow synthesis. Ketoreductase-catalyzed
asymmetric reduction of prochiral ketones is one of the most effective methods for the synthesis of chiral
alcohols. This paper summarizes the research progress of immobilized ketoreductase in continuous flow
synthesis, and provides guidance for developing efficient, economic, and sustainable flow biocatalysis
approaches.