JIA An, YANG Yi-Fang*, KONG De-Yun
.
2012, 43(4):
263-267.
The anti-inflammatory activity fractions were isolated from Callicarpa kwangtungensis Chun. and purified by column chromatographies, such as AD-8 macroporous resin, Sephadex LH-20, C18 and silica gel. Their structures were identified on the basis of physiochemical properties and spectroscopic methods (1H NMR, 13C NMR, HMBC and ESI-MS). Five C-glycosylflavones were isolated and identified as 5,7,3',4'-tetrahydroxy-8-C-β-Dglucopyranosyl
flavone(Ⅰ), 5,7,3',4'-tetrahydroxy-6-C-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranosyl flavone (Ⅱ), 5,7,3',4'-tetrahydroxy-8-methoxy-6-C-β-D-glucopyranosyl flavone(Ⅲ), 5,4'-dihydroxy-6,7-dimethoxy-8-C-[β-Dxylopyranosyl-( 1→2)]-β-D-glucopyranosyl flavone(Ⅳ) and 5,4'-dihydroxy-6,7-dimethoxy-8-C-β-D-glucopyranosyl flavone(Ⅴ). CompoundⅠwas isolated from Callicarpa kwangtungensis Chun. for the first time while the others were found from Callicarpa for the first time. The preliminary anti-inflammatory activities of the five compounds were investigated. The results showed that compounds Ⅰ, Ⅱ and Ⅲ had significant in vitro anti-inflammatory activity.