MA Yuhua1, WANG Hong2, SUI Hong2,3,4 , WANG Wenping2,3,4*
Bovine serum albumin (BSA) or BSA-Zn complex was dissolved or dispersed in poly (L-lactic acid) (PLLA) solution, which were further loaded into microparticles by emulsion-solvent evaporation method or hydrogel template technique (with polyvinyl alcohol as a template). Then four kinds of microparticles were obtained and their properties were characterized. The average particle size of the former two (named as s/o/w and w/o/w microparticles)
and the last two (named as s/o/t and w/o/t microparticles) were 38.13, 34.65, 49.47 and 45.82 μm, the span values were 0.77, 0.86, 0.22 and 0.26, the entrapment efficiencies were 15.62％, 7.74％, 85.87％ and 81.77％, respectively. The drugloading capacities of the two kinds of microparticles prepared by the former method were significantly decreased. The results of in vitro release test showed that the burst release at the first day of the above four products were 1.3％, 13.3％, 18.9％ and 55.7％, the cumulative release at 127 d were 14.1％, 83.6％, 57.5％ and 60.6％, respectively. It indicated that morphology and structure of microparticles significantly affected by the preparation methods, which further impacted drug-loading efficiency and release profile.
