The liposomes loaded with total flavones of Artemisia capillaris Thunb. were prepared by ethanol injection method, and N-trimethyl chitosan with positive charge was used to coat on the liposomes. Then, lyophilization was adopted to enhance the storage stability of the prepared liposomes. The results of single factor tests showed that lyophilized liposomes with good appearance and reconstitution profiles were obtained by using 6％ of trehalose as a lyoprotectant. The encapsulation efficiency, particle size, polydispersity index(PDI) and ζ potential of the liposomes before and after lyophilization were (91.8±0.5)％ and (92.5±0.3)％, (164.0±4.2) and (165.5±5.9)nm, 0.255±0.007 and 0.361±0.030, (23.8±0.2) and (21.6±0.4)mV, respectively. Under the transmission electron microscope, the reconstituted liposomes were spherical in shape. Furthermore, the liver protection effect of the liposomes was investigated with carbon tetrachloride-induced acute liver injury in murine models. Compared with model group, the levels of alanine aminotransferase(ALT), glutamic-oxaloacetic transaminase(AST) in serum and malonaldehyde in liver tissue of the liposomes treated group were significantly reduced, which demonstrated the title liposomes had a remarkable protective effect on acute hepatic injury in mice. This study can provide a new idea for the oral delivery of traditional Chinese medicine components with low solubility.