Determination of Chlorogenic Acid, Isofraxidin, Rosmarinic Acid and Picfeltarraenin IA in Yinjie Liyan Hanpian by Wavelength Switching-HPLC

TANG Dezhi

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主办:上海医药工业研究院
   中国药学会
   中国化学制药工业协会
ISSN 1001-8255   CN 31-1243/R   ZYGZEA
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Chinese Journal of Pharmaceuticals ›› 2016, Vol. 47 ›› Issue (11) : 1454. DOI: 10.16522/j.cnki.cjph.2016.11.024

Determination of Chlorogenic Acid, Isofraxidin, Rosmarinic Acid and Picfeltarraenin IA in Yinjie Liyan Hanpian by Wavelength Switching-HPLC

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Abstract

An HPLC method combined with wavelength switching technique was established for the determination of chlorogenic acid, isofraxidin, rosmarinic acid and picfeltarraenin IA in Yinjie Liyan Hanpian. An Agilent TC-C18 column was used, with the mobile phase of acetonitrile (A)∶0.2% phosphoric acid (B) by gradient elution, at the switching detection wavelength of 327 nm (0—12 min) for chlorogenic acid, 342 nm (12—25 min) for isofraxidin and rosmarinic acid, 264 nm (25—50 min) for picfeltarraenin IA. The method was found to be linear for the above four compounds in the ranges of 6.0—108.0, 1.0—18.0, 1.4—24.5 and 0.9—16.6 μg/ml, respectively. Their average recoveries were 99.85%, 99.31%, 99.46% and 99.30%, with RSDs of 1.4%, 1.6%, 1.1% and 1.4%, respectively.

Key words

Yinjie Liyan Hanpian / chlorogenic acid / isofraxidin / rosmarinic acid / picfeltarraenin IA / HPLC / content determination

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TANG Dezhi. Determination of Chlorogenic Acid, Isofraxidin, Rosmarinic Acid and Picfeltarraenin IA in Yinjie Liyan Hanpian by Wavelength Switching-HPLC. Chinese Journal of Pharmaceuticals. 2016, 47(11): 1454 https://doi.org/10.16522/j.cnki.cjph.2016.11.024

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