Paper
ZHANG Ying, XU Xiaqing, HE Meng, ZHANG Yueli, YANG Xiaorui, ZHANG Zhenzhong, GUO Xinhong
The photosensitive liposomes co-loaded with docetaxel(1) and fullerene C60 malonic acid derivative(2), a photosensitizer, were constructed by thin film dispersion method. The particle size, ζ potential, appearance, encapsulation efficiency, in vitro release, cytotoxicity of the prepared liposomes(1-2-LP) were characterized. The effects on reactive oxygen species(ROS) expression, cell cycle and apoptosis of the tumor cells, and in vivo antitumor activity in mice were also performed. The results showed that the particle size and ζ potential of the 1-2-LP were (170.4±1.3)nm and (–31.28±1.09)mV. The observation of transmission electron microscopy revealed that the product was quasi-spherical and uniform. The encapsulation efficiencies of 1 and 2 were (86.38±0.99)％ and (93.81±1.06)％. The release of 1 from the 1-2-LP was unaffected by the addition of 2, and the cumulative release of 1 within 50 h was 78％. The 1-2-LP could significantly inhibit the proliferations of human cervical cancer(HeLa) cells and mouse melanoma(B16-F10) cells under the irradiation of laser at 532 nm for 3 min. The detection results of ROS showed that 1-2-LP enhanced the fluorescence intensities of HeLa and B16-F10 cells under the laser irradiation. The results of flow cytometry showed the 1-2-LP combined with laser irradiation changed the cell cycle of the tumor cells and promoted their early apoptosis. The in vivo test in B16-F10 tumor-bearing mice showed that the tumor growth of the mice in 1-2-LP combined with laser irradiation group was significantly inhibited with the inhibitory rate of 85.33％, showing a synergistic antitumor effect of the liposomes co-loaded with 1 and 2 in combination with laser irradiation.
