Paper
WANG Yanquan, LI Cailin, NAJIB Mohammerd, PENG Fang, WU Dingyu
The effect and mechanism of periplaneta American polypeptide PAE2 in reversing the multi-drug resistance of hepatocellualar carcinoma resistant BEL-7402/5-FU cells were studied in this paper. Hepatocellualar carcinoma sensitive cells of BEL-7402, hepatocellualar carcinoma resistant 5-fluorouracil(5-FU) cells of BEL-7402/5- FU and Balb/c-nude mice were used as the research objects. MTT method was used to detect the multi-drug and crossdrug resistance of BEL-7402/5-FU cells, and the accumulation of doxorubicin in BEL-7402/5-FU cells was observed by laser confocal microscope. In order to determine the mechanism of the periplaneta American polypeptide PAE2 in reversing multi-drug resistance, real-time fluorescent quantitative polymerase chain reaction and immunocytochemical staining method were used to detect the mRNA and protein relative expression of P-glycoprotein 1(P-gp1), multi-drug resistance protein 1(MRP1), lung resistance protein 1(LRP1) and breast cancer resistance protein 1(BCRP1) in BEL- 7402/5-FU cells. In addition, an axillary tumor model of BEL-7402/5-FU cells was established in Balb/c-nude mice to observe the effects of periplaneta American polypeptide PAE2 on immune organs, liver and kidney function, tumor inhibition and induction of tumor cell apoptosis, as well as regulation of multi-drug resistance related proteins in tumor tissues. The results showed that the periplaneta American polypeptide PAE2 could effectively reverse the multi-drug and cross-drug resistance of BEL-7402/5-FU cells. The mechanism might be related to the down-regulation of the expression of multi-drug resistance related proteins, which reduced the efflux ability of multi-drug resistant cells on anti-tumor drugs and intervene molecular metabolism of anti-tumor drugs, reversing the multi-drug resistance of hepatocellualar carcinoma finally.