Paper
WANG Mengya, ZU Xiangyang, CHEN Fan, ZHAO Zhanqin, XUE Yun
The chitosan nanoparticles(CS-NPs) were prepared by ion cross-linking method, and the preparation parameters were optimized with mean diameter and polydispersion index(PDI) as indexes. The protective antigen protein Rv0180c (GLDKNKFDIRVVSPDEARRLY) in Mycobacterium tuberculosis H37Rv was synthesized according to the results of literature search. Then, the fluorescein isothiocyanate(FITC) labelled protein was encapsulated into the optimal blank CS-NPs to obtain the title nanoparticles(Pep-CS-NPs). The mean diameter, PDI and ζ potential of the blank CS-NPs were (148.13±2.24)nm, 0.197±0.013 and (+18.00±0.89)mV, respectively. No significant changes in particle size and size distribution of blank CS-NPs stored at 4 ℃ for 28 d were observed. The mean diameter, PDI, ζ potential, encapsulation efficiency and drug loading of the Pep-CS-NPs were (186.93±8.80)nm, 0.254±0.014, (+12.07±1.68)mV, (45.20±2.95)% and (12.92±1.12)%, respectively. In vitro release results showed that Pep-CS-NPs slowly released peptides and the cumulative release at 48 h was 56.6%. It was concluded that the process of preparing peptide-loaded CS-NPs by ion cross-linking method was simple, stable and mild, and the product had suitable particle size, uniform distribution and sustained-release profile, which was expected to be a new vaccine for treating tuberculosis.