ZHANG Yanyan1, HAN Guangming1, LI Weigang1, LUO Zhaoliang2
An HPLC method for the simultaneous determination of six furocoumarine components including psoralen, 8-methoxypsoralen, isopsoralen, 5-methoxypsoralen, imperator and trimethylpsorale in the Psoralea corylifolia Linn. from different habitats. A Capcell Pak-C18 column was used, with the mobile phase of methanol∶water for gradient elution, at the detection wavelength of 248 nm. The calibration curves were linear for the six components in the range of 1—100 μg/ml. Their average recoveries were 97.2％, 99.5％, 96.8％, 99.2％, 99.6％ and 98.7％, with RSDs of 1.54％,1.35％, 0.65％, 0.43％, 0.18％ and 0.26％, respectively. Contents of the six components in Psoralea corylifolia Linn. from ten different habitats were 0.21％—0.86％, 0.10％—0.17％, 0.28％—0.89％, 0.14％—0.43％, 0.10％—0.21％ and 0.10％—0.26％, respectively. The methodology validation proved that the reported method was applicable for the quality control of Psoralea corylifolia L..
