HU Tianyu, LI Hua, MA Jing*
2013, 44(2): 166-170.
Seventy SD rats were randomly divided into the following 5 groups: blank control, bleomycin (5 mg/kg) and oleic acid (12, 60 and 120 mg/kg) groups. All test animals were administered intranasally at a single dose. Their respiratory rate(RR), tidal volume(TV) and minute volume(MV) were recorded by computer at 0.5, 1, 2, 4 and 8 h after administration. The animals were sacrificed in three batches on d3, d7, d21. The respiratory toxicity effect of oleic acid was evaluated according to total and classified inflammatory cell count, protein concentration and lactate dehydrogenase (LDH) activity in the bronchoalveolar lavage fluid (BALF) of the left lobe of lung. The hydroxyproline (HYP) content in right upper lobe of lung was determined and the histopathologic changes of right lower lobe were
observed. The results showed that the RR level at 0.5 h of three oleic acid groups were obviously higher than that of blank control group, while the TV level were lower. Compared with the blank control group, the total number of inflammatory cells, neutrophilic granulocyte, eosinophilic granulocytes, protein concentration and LDH activity in BALF in high dose group of oleic acid were significantly increased and lung section showed inflammatory response and numerous foam cells. There were no significant differences between three oleic acid groups and blank control group in HYP content. It indicated that high dose of oleic acid had an acute injury on rat lung tissue and affected the respiratory function, but this effect was normally reversible.